Immunohistochemical Analysis of Brain from G2 Mice Inoculated with TSE Strains ME7, 79A, and 301C

<div><p>Brain sections were immunostained for PrP using monoclonal antibody 6H4 and analysed by light microscopy using a Nikon Eclipse E 800 microscope. Brains from clinically positive G2 mice inoculated with (A) ME7 or (B) 79A showing wide spread PrP deposition. Brains from 129/Ola wild-type mice inoculated with (C) ME7 or (D) 79A also showing wide spread PrP deposition.</p> <p>The TSE strain 301C was used to inoculate (E and F) G2 mice, which showed wide spread plaque-like and fine punctuate PrP deposits in the cerebrum and cerebellum or (G and H) 129Ola mice, which also showed plaque-like PrP deposition. All 301C inoculated brains shown were from clinically positive mice.</p> <p>Magnifications: (A–E) and (G), 4×; (F) and (H), 10×.</p>