Lesion Profile Analysis of TSE-Inoculated PrP Glycosylation Mutant Mice

<div><p>G2 and 129/Ola mice were inoculated i.c. with TSE strains ME7 (A), 79A (B), or 301C (C) and G1 mice were inoculated i.c. with 79A (D). Squares: wild type; open triangles: G2; and solid triangles: G1. All mice (12 in each group) used in these analyses were clinically and pathologically positive.</p> <p>Nine gray matter areas: 1, dorsal medulla; 2, cerebellar cortex; 3, superior colliculus; 4, hypothalamus; 5, medial thalamus; 6, hippocampus; 7, septum; 8, cerebral cortex; 9, forebrain cortex and three white matter areas: 1*, cerebellar white matter; 2*, mesencephalic tegmentum; 3*, pyramidal tract (<i>x</i>-axis) were scored on scales of 0–5 for gray and 0–3 for white matter areas (<i>y</i>-axis). The mean scores for each area are shown (error bars ± SEM). No differences between wild-type and G2 mice were observed after inoculation with ME7 (A) or 79A (B); however some differences in the superior colliculus and white matter areas were observed between the two groups when inoculated with 301C (C). Differences were observed between wild-type and G1 mice inoculated with 79A (D) especially in area: 2, 4, 8, and in the white matter.</p>