Western Blot Analysis of Brains from TSE Inoculated Glycosylation Mutant Mice

<div><p>Brain homogenates were treated with or without PK as indicated prior to SDS PAGE analysis.</p> <p>(A) G3 and wild-type animals inoculated with 79A. Lanes 1–2: 129/Ola wild-type mice clinically (clin) positive/pathologically (path) positive; lanes 3–4: G3 mice clin positive/path positive; lanes 5–6: G3 mice clin negative/path positive.</p> <p>(B) G3 and wild-type animals inoculated with ME7. Lanes 1–2: 129/Ola mice clin positive/path positive; lanes 3–4: G3 mice clin negative/path negative with no plaques; lanes 5–6: G3 mice clin negative/path negative with plaques.</p> <p>(C) G2 and wild-type animals inoculated with ME7. Lanes 1–2: 129/Ola; lanes 3–6: G2 mice, brains positive for plaques. All animals clin positive/path positive.</p> <p>(D) G2 and wild-type animals inoculated with 79A. Lanes 1–2: 129/Ola mice; lanes 3–6: G2 mice. All animals clin positive/path positive.</p> <p>(E) G2 and wild-type animals inoculated with 301C. Lanes 1–2: 129/Ola mice; lanes 3–6: G2 mice brains positive for plaques. All animals clin positive/path positive.</p> <p>(F) G1 and wild-type animals inoculated with 79A. Lanes 1–2: 129/Ola mice; lanes 3–6: G1 mice. All animals clin positive/path positive. Lanes 1–4: 3-min exposure; lanes 5–6: 10-min exposure.</p> <p>Molecular weight markers indicated in KDa. Wild-type (Wt) mice are 129/Ola mice. Monoclonal antibody 7A12 (kindly provided by M.-S. Sy) was used for immunodetection of PrP.</p>