Western Blot Analysis of Brains from Second Passage of 79A-Infected G2 and Wild-Type Mice

<div><p>Brain homogenates from (A) clinically and pathologically positive 79A inoculated G2 or wild-type, 129/Ola (Wt), mice were used to i.c. inoculate 129/Ola mice.</p> <p>(B) The 79A inoculated G2 mouse brains lacked diglycosylated PrP^Sc, but all 129/Ola mice inoculated with 79A/G2 or 79A/Wt brain material had di-, mono-, and unglycosylated PrP^Sc.</p> <p>(C) Brain of a C57Bl mouse infected with 79A used as comparison for the glycotype.</p> <p>Brain homogenates from (D) clinically and pathologically positive ME7 inoculated-G2 or wild-type, 129/Ola (Wt), mice were used to intracerebrally inoculate 129/Ola mice.</p> <p>(E) The ME7 inoculated G2 mouse brains lacked diglycosylated PrP^Sc but all 129/Ola mice inoculated with ME7/G2 or ME7/Wt brain material had di-, mono-, and unglycosylated PrP^Sc.</p> <p>(F) Brain of a C57Bl mouse infected with ME7 used as comparison for the glycotype.</p> <p>Lanes 1–6: TSE-inoculated 129/Ola mouse brain. All samples were PK treated.</p>