Neuropathological changes and PrP^Sc distribution in hippocampal and cerebellar sections of prion-challenged mice.

<p>(<b>A</b>) First column: Paraffin-embedded tissue (PET) blots demonstrating PrP^Sc deposits in hippocampus and cerebellum of RML-infected wild-type (C57/129Sv) and PrP(TetraH>G), line 34 mice. There are plaque-like deposits in and adjacent to the corpus callosum and the internal granular cell layer of the cerebellum in PrP(TetraH>G) mice. Second column: Corresponding PrP^Sc immunhistochemistry (upper field: hippocampus and corpus callosum; lower field: cerebellum; monoclonal antibody CDC1; scale bar: 50 μm). Third column: Hematoxylin and eosin stainings (H&E) of hippocampal/corpus callosum and cerebellar sections demonstrating spongiform changes (scale bars of hippocampus/corpus callosum: 100 μm; of cerebellum: 200 μm). Fourth column: GFAP immunostaining demonstrating gliosis (scale bars as in third column). (<b>B</b>) Corresponding histopathological and immunohistochemical examination of hippocampal brain sections derived from terminally ill PrPH95G mice and corresponding wt controls (scale bars: 200 μm). Three independent Tg lines (lines 4, 11 and 13) were assessed for these analyses.