Neuropathological analysis of transgenic mouse brain.

<p>Panels A, E, I and M, schematics showing regional distribution of abnormal PrP deposits. Note that these panels reflect the overall spatial distribution of neuropathology and are not meant to indicate precise representations of individual brains. Panels B, F, J and N, H&E staining demonstrating spongiform neurodegeneration in the hippocampal areas. Panels C, G, K and O, PrP immunohistochemistry using anti-PrP monoclonal antibody ICSM 35 demonstrates abnormal PrP immunoreactivity. Panels D, H, L and P, GFAP staining demonstrating gliosis in the hippocampal areas. A–D, IPD A117V prions inoculated to 117VV Tg31 mouse; E–H, sporadic CJD prions inoculated to 117VV Tg31 mouse showing distinctive diffuse synaptic PrP deposition characteristic of sCJD prions (panel G); I–L, IPD A117V prions inoculated to 117VV Tg30 mouse. M–P, vCJD prions inoculated to 117VV Tg30 mouse showing abundant non-florid PrP plaques (panel O). Scale bar = 500 µm for all panels except A, E, I and M.