Detection of disease-related PrP by immunohistochemistry and immunoblotting in brains of transgenic mice inoculated with GSS-102L prions.

<p>The anti-PrP monoclonal antibodies used were, ICSM 18 (panels A, C, E, G) that recognizes both human PrP 102L and wild type human PrP and ICSM 35 (panels B, D, F, H) that recognizes wild type human PrP but not human PrP102L. The provenance of each brain sample analysed by immunoblotting is designated above each lane in panels A and B. (A, B) IPD P102L patient brain homogenate (P102L) in comparison to primary and secondary transmission into transgenic mice. All transgenic lines designated above each panel (C, E, G) were inoculated with inherited prion disease <i>PRNP</i> P102L 129 MM (IPD P102L) prions previously passaged in 102LL Tg27 transgenic mice. Only 102LL Tg27 transgenic mice were susceptible to the homogeneous 102L prions. Scale bar, 160 μm in all images.