Vacuolation and PrPS^c deposition in the brain is the same in mice infected with 22L or 22L plus FV.

<p>(A–C) Hemotoxylin and eosin staining of the cerebellum from (B10 X A.BY)F1 mice inoculated (A) i.v. with FV alone, (B) i.p. with mouse 22L mouse scrapie alone, or (C) co-infected with 22L mouse scrapie i.p. and FV i.v.. Magnification = 200×. (D–F) PrP^Sc deposition in the cerebellum of (B10 X A.BY)F1 mice inoculated (D) i.v. with FV alone, (E) i.p. with mouse 22L mouse scrapie alone, or (F) co-infected with 22L mouse scrapie i.p. and FV i.v.. Sections were stained using the anti-PrP mouse monoclonal antibody D13 following hydrated autoclaving at high pressure and temperature. Red stain indicates the presence of PrP^Sc. Magnification = 200×.