Infection with iRBCs induces alterations on Tregs and APCs inside the liver.

<p>Foxp3-GFP or CD11c-YFP mice were intraperitoneally infected or not with Pc-iRBCs. All animals were divided into three groups: Control (non-infected; open columns); infected, on day 7 of infection (INF d7; grey columns); or infected, on day 21 of infection (INF d21; black columns). Liver cells were stained with MAbs against a panel of surface molecules to identify different types and subtypes of leukocytes, as well as the expression levels of some proteins. (A) Numbers and percentages of Foxp3+CD4+ cell (Tregs) in the liver samples. (B) CD28 and GITR expression levels on Tregs. (C) Percentages of lymphoid (LDCs) or myeloid (mDCs) DCs in the liver samples. (D) MHCII, CD86, and F4/80 expression levels on DC subtypes and macrophages. These results are representative of 3 repetitions.