Expansion of CD4⁺Foxp3⁺ Tregs in the liver of <i>aly/aly</i> mice during persistent <i>L. donovani</i> infection.

<p>Flow cytometry analysis of CD4⁺Foxp3⁺ and CD8⁺Foxp3⁺ Tregs among the hepatic lymphocytes. (A) Representative data of the gated lymphocytes extracted from the liver at 12 WPI of <i>aly/+</i> (left) and <i>aly/aly</i> mice (right), stained with anti-Foxp3 and anti-CD4 or anti-CD8 mAb. (B) The proportion of CD4⁺Foxp3⁺ T cells to CD4⁺ T cells in <i>aly/+</i> (open bars) and <i>aly/aly</i> (filled bars) mice. (C) The total number of CD4⁺Foxp3⁺ T cells in <i>aly/+</i> (open bars) and <i>aly/aly</i> (filled bars) mice at 8–16 WPI. A typical result of two individual experiments is shown. Data are the mean ± SE for three mice of each strain. ND, not determined; * <i>p</i><0.05; *** <i>p</i><0.001.