<i>In vitro</i> suppression capacity of liver Tregs after infection with iRBCs.

<p>Foxp3-GFP mice were intraperitoneally infected (or not) with Pc-iRBCs. All animals were divided into two groups: Control (non-infected) or infected, on day 7 of infection (INF d7). (A) Conventional CD4+Foxp3- T cells (Tconv) were isolated from INF d7 liver (Liver 7d) or spleen (Spleen 7d) samples or from non-infected spleens (Spleen 0d) by flow cytometry. Next, these cells were labeled with VioletCell tracer to measure proliferation by tracer dilution among cells. Note that Tconv from infected animals did not proliferate after <i>in vitro</i> anti-CD3 stimulation; proliferation was observed in non-infected Tconv only. (B) CD4+Foxp3+ T cells (Tregs) isolated from the same animals described above were co-cultured with APCs and naïve Tconv, with anti-CD3 stimulation. The histograms show the VioletCell tracer dilution after culture. (C) Percentages of naïve Tconv cells that proliferated with different concentrations of Tregs in culture. These results are representative of 3 independent experiments.