(A) Hematoxylin and eosin staining of liver sections obtained from CYP2D6 and FVB/N mice infected with either Ad-2D6 or Ad-GFP at weeks 1, 4, and 8 after infection

Note that persistent hepatic damage (massive cellular infiltrations and significant necrosis) is present in Ad-2D6–infected mice only. Infection of FVB/N mice with Ad-GFP results in a similar histology as Ad-GFP infection of CYP2D6 mice (not depicted). Bars, 50 μm. (B) The following scoring system was used to assess liver infiltration: 0, no visible infiltration; 1, few focal infiltrates; 2, numerous scattered focal infiltrates; 3, few large clusters of infiltrating cells (perivascular or subcapsular); 4, numerous large clusters of infiltrating cells occupying >50% of portal tracts or central veins; and 5, confluent periportal infiltrates. The presented data are mean infiltration scores ± SEM. Statistical analysis (Mann-Whitney test) revealed significant differences between mice infected with Ad-2D6 or Ad-GFP at the indicated times after infection. *, P < 0.05. The number of individual mouse livers analyzed per experimental group is indicated at the bottom of each data column. (C) Immunohistochemistry of liver sections of wild-type FVB/N and humanized CYP2D6 mice at week 2 after infection with Ad-2D6. Cellular infiltrations consisted predominantly of B cells (B220), CD4 T cells, and macrophages (F4/80). Bars, 50 μm.<p><b>Copyright information:</b></p><p>Taken from "Breaking tolerance to the natural human liver autoantigen cytochrome P450 2D6 by virus infection"</p><p></p><p>The Journal of Experimental Medicine 2008;205(6):1409-1422.</p><p>Published online 9 Jun 2008</p><p>PMCID:PMC2413037.</p><p>