Evaluation of cellular infiltrate present in the liver as consequence of AdTk/GCV mediated liver injury.

<p>Control, AdTk alone (AdTk) or treated with GCV (AdTk+GCV) mice were sacrificed at different time points after GCV administration. The livers were collected and immunohistochemistry for leukocytes (CD45 antigen) (A), oval cells (B) and activated stellate cells (C). Sirius Red staining was used for characterizing collagen accumulation (D). Representative examples of immunohistochemically stained sections are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0074948#pone.0074948.s001" target="_blank">Figure S1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0074948#pone.0074948.s001" target="_blank">Figure S2</a> in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0074948#pone.0074948.s001" target="_blank">File S1</a>. Three stained liver sections from 5 animals/time point/group were quantified using FIJI imaging software and obtained results were normalized with control group values. X axis represents the days post GCV administration and liver damage induction while Y axis represents normalized ratio between positive area and total area in arbitrary units. Groups were compared at each time point (* <i>p</i>< 0.05, ** <i>p</i>< 0.01, *** <i>p</i>< 0.001, two-tailed Student’s <i>t</i>-test). Results obtained from mice treated with GCV alone are similar to the observed in control and AdTk mice.