Effects of anti-Treg antibodies on Foxp3 expression, parasite burden and infected foci in the liver.

<p>The <i>L. donovani</i>-infected <i>aly/aly</i> mice were intraperitoneally injected with anti-CD25 or anti-FR4 mAb at 26 WPI and hepatic immune responses were examined after 10 days of injection. (A) <i>Foxp3</i> mRNA expression by RT-PCR. (B) Parasite burden expressed by qPCR. (C) The number of infected foci from 25 consecutive microscopic fields. (D) Frequency of mature (black column) and immature (grey column) granuloma, and no response (white column) in the infected foci. (E) Localization of Foxp3-stained cells in the liver parenchyma including “Immature granulomas” and “Mature granulomas”. The brown pigments represent Foxp3-immunoreactive cells. * <i>p</i><0.05; *** <i>p</i><0.001.