<i>Ahnak</i>-KO mice displayed reduced adiposity.

<p>(<b>A</b>) AHNAK expression of obese human subjects. (<b>B</b>) Changes in body weights during 12 weeks of feeding on RC or an HFD; <i>n</i> = 3 for WT and KO mice on regular chow diet (RC), <i>n</i> = 4 for WT and KO mice on HFD. (<b>C</b>) Body-composition changes in mice after 4 weeks on an HFD; WT: <i>n</i> = 6, KO: <i>n</i> = 5. (<b>D</b>) Representative pictures of fat pads from mice fed an HFD. (<b>E</b>) H&E staining (left) of eWAT and distribution of adipocyte sizes (right); <i>n</i> = 3 Scale bar, 200μm. (<b>F</b>) Relative mRNA levels of the indicated genes in WATs were measured by qPCR. Values were normalized to <i>36B4</i>. WT: <i>n</i> = 5, KO: <i>n</i> = 8 (<b>F</b>). The data shown are the mean±SEM. *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001 between WT and KO mice. eWAT, epididymal white adipose tissue.