Characterization of obese conditions among each experimental group.

<p>WT or AD transgenic mice were treated with NCD or HFSTZ (A) Body weights were recorded by week. (B) Epididymal fat tissue was collected, fat mass estimation based on the percentage of epididymal fat of total body weight at after 11 weeks of dietary manipulations. (C) Representative histological microphotographs of HE-stained epididymal fat sections (scale bar, 50 μm). Adipocyte size was measured by ImageJ. (D) Serum leptin and (E) free fatty acid were quantified by ELISA after 11 weeks of dietary manipulation. Bars represent the mean ± SEM of at least three independent experiments. Experimental groups labeled with different letters are significantly different from each other (<i>p</i> < 0.05).