Characterization of the diet-induced obese phenotype.

<p>C57BL/6 mice were fed a high diet (HF), high fat diet supplemented with FO (HF-FO) or a low fat (LF) control diet for 12 weeks (n = 12−17 TNBS-treated and 4–6 vehicle controls/diet). Mice were presensitized with 1% TNBS or vehicle control (week 11), followed by a 2.5% TNBS enema or vehicle control (week 12) and sacrificed 3 d post-TNBS. A) Changes in body weight over time. B) Visceral adipose tissue weight from individual visceral depots (perinephric, mesenteric and epididymal) or combined (total visceral adipose). Serum concentrations of C) insulin, D) leptin, E) resistin and F) adiponectin. All data were analyzed by two-way ANOVA (main effects: diet and treatment) and <i>P</i>-values are shown. Bars represent means ± SEM and statistical significance was (<i>P</i>≤0.05). Panel A) asterisk (*) indicates statistically significant time points where the HF and HF-FO groups differed from LF (<i>P</i>≤0.05). Panels B-F) bars not sharing a common letter differ (<i>P</i>≤0.05).