HFD feeding leads to obesity and onset of adipose tissue inflammation.

<p>APOE*3Leiden transgenic mice were fed a HFD for 12 weeks and sacrificed at the time points indicated. The average body weight at the start (t=0) of HFD feeding was 29.2 g. <b>A</b>, Body weight gain over time. <b>B</b>, Mass of the epididymal adipose tissue depot during obesity development. Data are presented as mean ± SEM. <b>C</b>, Histological analysis of adipose tissue at start (t=0) and after 12 weeks of HFD or chow feeding (reference for the effect of aging). <b>D</b>, HFD feeding results in adipocyte hypertrophy. Computer-assisted quantification of average adipocytes size (P<0.05). <b>E</b>, Marked accumulation of CCR2 positive cells (arrows) in the HFD fed group.