The Development of Diet-Induced Obesity and Glucose Intolerance in C57Bl/6 Mice on a High-Fat Diet Consists of Distinct Phases - Figure 12

<p><b>A.</b> The area of F4/80 staining in the WAT measured as arbitrary units (AU) was increased in HF fed mice at 12 and 16 weeks compared to the LF diet and almost reached significance at 16 weeks (<i>P</i> = 0.058). <b>B.</b><i>IL-1β</i> gene expression in white adipose tissue (WAT) was slightly up-regulated after 3 days of HF diet but was also below the level considered to be significant (<i>P</i> = 0.06) and was unchanged at 1 week and up-regulated at 16 weeks (<i>P</i><0.05). <b>C.</b><i>IL-6</i> gene expression was up-regulated after 3 days on a HF diet (<i>P</i><0.05), unchanged after 1 week and marginally up-regulated at 16 weeks (<i>P</i> = 0.054). <b>D.</b><i>TNFα</i> gene expression was unchanged after 3 days and 1 week on HF diet but was up-regulated at 16 weeks (<i>P</i><0.001). <b>E.</b><i>CXCL-1 gene</i> expression was unchanged at 3 days and 1 week on a HF diet but was up-regulated after 16 weeks (<i>P</i><0.001). <b>F.</b><i>F4/80</i> gene expression was unchanged after 3 days and 1 week on a HF diet and up-regulated 16 weeks (<i>P</i><0.01) <b>G.</b><i>MRC-1</i> gene expression was unchanged after 3 days and 1 week of HF diet but was up-regulated 16 weeks (<i>P</i><0.01) <b>H.</b><i>IL-10</i> gene expression was unchanged after 3 days and 1 week on a HF diet and was up-regulated at 16 weeks (<i>P</i><0.01) <b>I.</b><i>ARG</i> gene expression was unchanged after 3 days and 1 week of HF diet but was up-regulated 16 weeks (<i>P</i><0.01). <b>J</b><i>ARG</i> gene expression in WAT is unchanged between 3 days and 1 week and up-regulated between 3 days and 16 weeks (<i>P</i><0.05) on a LF diet. <b>K.</b><i>IL-6</i> gene expression in WAT is unchanged between 3 days and 1 week and up-regulated between 3 days and 16 weeks on a LF diet (<i>P</i><0.01). <b>L.</b><i>IL-1β</i> gene expression in WAT is unchanged between 3 days and 1 week and up-regulated between 3 days and 16 weeks (<i>P</i><0.01) on a LF diet. Units are fold expression changes between experimental groups relative to <i>B2M</i> and calculated from the ΔΔ<i>C</i>_t values (n = 6–8).