Increased adiposity in Gal-3 KO mice.

<p>Parameters of adiposity were evaluated in Lean WT (yellow), Lean Gal-3 KO (red), DIO WT (green) and DIO Gal-3 KO (blue) mice. Body weight (<b>A</b>) was evaluated weekly. Food intake (<b>B</b>) was evaluated weekly and average daily food intake per mouse is shown. Absolute fat mass by DXA (<b>C</b>), plasma leptin (<b>D</b>) and APN (<b>J</b>) levels as well as median adipocyte size (µm²) in VAT (<b>E</b>) and SAT (<b>F</b>) were measured at week 20, together with plasma TG levels (<b>G</b>). Expression of mRNA for APN in VAT (<b>H</b>) and SAT (<b>I</b>) as well as Gal-12, ATGL and PPARγ in VAT (<b>M</b>) was evalated by qPCR, also at week 20. Data in panels H, I and M are presented as fold difference <i>versus</i> Lean WT mice after normalization for expression of GAPDH. Release of leptin (<b>K</b>) and APN (<b>L</b>) from cultures of VAT <i>ex vivo</i> was measured by ELISA. Data are expressed as pg of adipokine/mg of VAT. Data are mean+/−SEM, n = 10. *p<0.05, **p<0.01 <i>versus</i> respective WT group; °p<0.05, °°p<0.01, °°°p<0.001 <i>versus</i> respective Lean group; ∧p<0.05,∧p<0.001 vs WT Lean.