Insulin and leptin resistance in wild-type B6 DIO mice.

<p>Changes in plasma free fatty acids (A) and insulin (B) before and after 4 and 7 days at 4°C in greater and lesser obese B6 mice. Correlation between fat mass and plasma leptin levels in DIO mice (C). Changes in plasma leptin (D) before and after 4 and 7 days at 4°C in DIO mice. Changes in food intake and body weight and composition (E) in mice administered with leptin at 24 and 4°C. Data are expressed as mean ± SEM. *, significant differences (<i>t</i> test, *, <i>P</i> < 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.005).