<p>Tubulin was pre-incubated for 1 min with <b>10s</b> at 2 μM, 10 μM, 25 μM and 50 μM, CA-4 at 0.5 μM, paclitaxel at 5 μM or vehicle DMSO (control) at room temperature before GTP was added to start the tubulin polymerization reactions. The reaction was monitored at 37°C.</p
<p>Black circle – General Tubulin Buffer (80 mM PIPES, MgCl<sub>2</sub>, 0.5 mM EGTA, pH 6.9). Green...
<p>Cells were treated with or without 2.5 μM MPT0B169 for 24 h. The polymer and free form of tubulin...
<p>(A) Soluble tubulin extraction assays were performed after treatment with serial dilutions of CA4...
<p>Purified bovine tubulin and GTP were mixed in a 96-well plate. The reaction was initiated by warm...
<p><b>A</b>) As a reference, 1 µM tubulysin A was added to a preparation of 10 µM tubulin, and the a...
<p>In vitro polymerization of tubulin, purified from pig brain, in the presence of 0μM (●), 0.25μM (...
<p>Effects of LJK-11 (250 µM), colchicines (10 µM), nocodazole (10 µM), or Taxol (10 µM) on bovine b...
<p>(A) Tubulin assembly study. Tubulin (12 µM) was polymerized separately in the presence of (0 −200...
<p>Tubulin was incubated with the compounds ((A), ESE-16 (B), ESE-15-one (C) and EMBS (D)), as descr...
The kinetic and thermodynamic behavior of in vitro taxol-induced polymerization of purified tubulin ...
<p>Purified tubulin polymerization was quantitated by turbidimetry measurement at 350 nm with or wit...
<p>(A) Tubulin (10 µM) was polymerized in the absence (▪) and presence of 10 (◊), 25 (▴), 50 (×), 75...
<p>PC12 cells were incubated with 10 μM ASN for 48 h. SB-216763, Colchicine and Taxol were used as G...
<p>A, Tubulin (10 µM) was polymerized in the absence and presence of 4 µM MD, CT, MDCT or MD and CT ...
<p>Tubulin was incubated at 37°C in the reaction buffer in the presence of (A) 1% DMSO (vehicle cont...
<p>Black circle – General Tubulin Buffer (80 mM PIPES, MgCl<sub>2</sub>, 0.5 mM EGTA, pH 6.9). Green...
<p>Cells were treated with or without 2.5 μM MPT0B169 for 24 h. The polymer and free form of tubulin...
<p>(A) Soluble tubulin extraction assays were performed after treatment with serial dilutions of CA4...
<p>Purified bovine tubulin and GTP were mixed in a 96-well plate. The reaction was initiated by warm...
<p><b>A</b>) As a reference, 1 µM tubulysin A was added to a preparation of 10 µM tubulin, and the a...
<p>In vitro polymerization of tubulin, purified from pig brain, in the presence of 0μM (●), 0.25μM (...
<p>Effects of LJK-11 (250 µM), colchicines (10 µM), nocodazole (10 µM), or Taxol (10 µM) on bovine b...
<p>(A) Tubulin assembly study. Tubulin (12 µM) was polymerized separately in the presence of (0 −200...
<p>Tubulin was incubated with the compounds ((A), ESE-16 (B), ESE-15-one (C) and EMBS (D)), as descr...
The kinetic and thermodynamic behavior of in vitro taxol-induced polymerization of purified tubulin ...
<p>Purified tubulin polymerization was quantitated by turbidimetry measurement at 350 nm with or wit...
<p>(A) Tubulin (10 µM) was polymerized in the absence (▪) and presence of 10 (◊), 25 (▴), 50 (×), 75...
<p>PC12 cells were incubated with 10 μM ASN for 48 h. SB-216763, Colchicine and Taxol were used as G...
<p>A, Tubulin (10 µM) was polymerized in the absence and presence of 4 µM MD, CT, MDCT or MD and CT ...
<p>Tubulin was incubated at 37°C in the reaction buffer in the presence of (A) 1% DMSO (vehicle cont...
<p>Black circle – General Tubulin Buffer (80 mM PIPES, MgCl<sub>2</sub>, 0.5 mM EGTA, pH 6.9). Green...
<p>Cells were treated with or without 2.5 μM MPT0B169 for 24 h. The polymer and free form of tubulin...
<p>(A) Soluble tubulin extraction assays were performed after treatment with serial dilutions of CA4...