Dose-response effects of CA-4 (1), 7i and 7k on the inhibition of tubulin polymerization.

<p>Purified bovine tubulin and GTP were mixed in a 96-well plate. The reaction was initiated by warming the solution from 4°C to 37°C. DMSO was used as a vehicle control. The effect on the assembly of tubulin was monitored using a plate reader at 1 min intervals for 90 min at 37°C.