BCFMT inhibited tubulin polymerization <i>in vitro</i>.

<p>(A) Tubulin (10 µM) was polymerized in the absence (▪) and presence of 10 (◊), 25 (▴), 50 (×), 75 (○) and 100 (□) µM BCFMT. (B) Electron micrographs of tubulin polymers in the absence and presence of 25 and 50 µM BCFMT. The scale bar is 2000 nm. (C) BCFMT suppressed the GTPase activity of microtubules. Effects of vinblastine on the GTPase activity of microtubules under similar experimental conditions are shown in the inset. Data were an average of three independent experiments. Bars represent ± SD.