Effects of MD, CT and MDCT on the assembly of purified tubulin.

<p>A, Tubulin (10 µM) was polymerized in the absence and presence of 4 µM MD, CT, MDCT or MD and CT in PEM buffer containing 1 mM GTP. Supernatant and pellet fractions were analyzed using 12% SDS-PAGE. B, Ratio of polymerized tubulin to soluble tubulin in the presence of different fragments is plotted as mean ± SD of three sets of experiments.