<p>PC12 cells were incubated with 10 μM ASN for 48 h. SB-216763, Colchicine and Taxol were used as GSK-3β inhibitor, microtubule destabilizer and microtubule stabilizer, respectively. The level of free and polymerized α/β-tubulin was determined using the Western blotting method. (<b>A</b>) Immunoreactivity of free and polymerized α/β-tubulin (<b>B</b>) Densitometric analysis of free and polymerized α/β-tubulin immunoreactivity. Microtubule instability expressed as a ratio of free to the polymerized tubulin. Data represent the mean value ± S.E.M. for 3 independent experiments. ** p<0.01 <i>versus</i> control; # p<0.01 <i>versus</i> ASN-treated cells using a one-way ANOVA followed by Dunnett's Multiple Comparison test.</p
<p>(<b>A</b>) Cultured neurons were untreated or treated with paclitaxel (Taxol; 20 µM) for 40 minut...
<p>Purified tubulin polymerization was quantitated by turbidimetry measurement at 350 nm with or wit...
<p>HeLa cells were treated with 0, 15, 30, 60, 125, 250 µg/ml amitozyn for 8 h, fixed and stained wi...
<p>In vitro polymerization of tubulin, purified from pig brain, in the presence of 0μM (●), 0.25μM (...
<p>(A) Soluble tubulin extraction assays were performed after treatment with serial dilutions of CA4...
<p>Cells were treated with or without 2.5 μM MPT0B169 for 24 h. The polymer and free form of tubulin...
<p>FACS, Western blot and cell growth are all from three independent experiments. (A) <i>T</i>. <i>b...
<p>(A) <i>In-vitro</i> tubulin polymerization assays were used to test the effect of the 212 mitotic...
<p><b>A</b>) As a reference, 1 µM tubulysin A was added to a preparation of 10 µM tubulin, and the a...
Abstract: Microtubules are important components of the cellular cytoskeleton that play roles in vari...
<p>(A) Tubulin assembly study. Tubulin (12 µM) was polymerized separately in the presence of (0 −200...
Tubulin is a protein that plays a critical role in maintaining cellular structure and facilitating c...
<p>Tubulin was incubated with the compounds ((A), ESE-16 (B), ESE-15-one (C) and EMBS (D)), as descr...
(1) Background: Microtubule depolymerizing agents (MDAs) are commonly used for cancer treatment. How...
<p>PC12 cells were incubated in the presence of 10 μM ASN for 48 h. The total level of GSK-3β was de...
<p>(<b>A</b>) Cultured neurons were untreated or treated with paclitaxel (Taxol; 20 µM) for 40 minut...
<p>Purified tubulin polymerization was quantitated by turbidimetry measurement at 350 nm with or wit...
<p>HeLa cells were treated with 0, 15, 30, 60, 125, 250 µg/ml amitozyn for 8 h, fixed and stained wi...
<p>In vitro polymerization of tubulin, purified from pig brain, in the presence of 0μM (●), 0.25μM (...
<p>(A) Soluble tubulin extraction assays were performed after treatment with serial dilutions of CA4...
<p>Cells were treated with or without 2.5 μM MPT0B169 for 24 h. The polymer and free form of tubulin...
<p>FACS, Western blot and cell growth are all from three independent experiments. (A) <i>T</i>. <i>b...
<p>(A) <i>In-vitro</i> tubulin polymerization assays were used to test the effect of the 212 mitotic...
<p><b>A</b>) As a reference, 1 µM tubulysin A was added to a preparation of 10 µM tubulin, and the a...
Abstract: Microtubules are important components of the cellular cytoskeleton that play roles in vari...
<p>(A) Tubulin assembly study. Tubulin (12 µM) was polymerized separately in the presence of (0 −200...
Tubulin is a protein that plays a critical role in maintaining cellular structure and facilitating c...
<p>Tubulin was incubated with the compounds ((A), ESE-16 (B), ESE-15-one (C) and EMBS (D)), as descr...
(1) Background: Microtubule depolymerizing agents (MDAs) are commonly used for cancer treatment. How...
<p>PC12 cells were incubated in the presence of 10 μM ASN for 48 h. The total level of GSK-3β was de...
<p>(<b>A</b>) Cultured neurons were untreated or treated with paclitaxel (Taxol; 20 µM) for 40 minut...
<p>Purified tubulin polymerization was quantitated by turbidimetry measurement at 350 nm with or wit...
<p>HeLa cells were treated with 0, 15, 30, 60, 125, 250 µg/ml amitozyn for 8 h, fixed and stained wi...