Co-localization of endogenous Dyrk1A and clathrin in the adult mouse brains.

<p>Forty micrometer sections of the mouse brain tissues were incubated with polyclonal anti-Dyrk1A (red, <i>a</i>, <i>d</i>, and <i>g</i>) and monoclonal anti-CHC (green, <i>b</i>, <i>e</i>, and <i>h</i>) antibodies as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034845#s2" target="_blank"><i>MATERIALS AND METHODS</i></a>. Presented are Z-stack images collected from the subiculum (<i>a–c</i>), the medio-posterior thalamic nucleus (<i>d–f</i>), and individual confocal images from the cerebellar Purkinje cell layer (<i>g–i</i>). Structures showing co-localization of Dyrk1A and CHC are visible on merged images (yellow in <i>c, f, i</i>). The dotted squares shown in <i>a–i</i> are enlarged in the upper right corners. Scale bar = 10 µm. Panels (<i>j–m</i>) show three consecutive scanning images from subiculum. Pearson's correlation coefficients for three consecutive scanning images from subiculum and the medio-posterior thalamic nucleus were calculated as 0.439 and 0.499, respectively, by using NIH Image J with the JACoP plug-in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034845#pone.0034845-Bolte1" target="_blank">[65]</a>.