Co-localization of clathrin and dynamin1 in PC12 cells.

<p>PC12 cells co-transfected with clathrin-dsRed (A,C) and dynamin1-EGFP (B,D) were imaged by TIR-FM. Regions drawn around clathrin spots (A) were transferred to dynamin1 images (red circles in D). Regions drawn around dynamin1 spots (B) were transferred to clathrin images (green circles in C). 250 spots per group were evaluated to determine if clathrin and dynamin1 co-localize (E). Dynamin1 at sites of clathrin spots is significantly higher than dynamin1 outside clathrin spots. Clathrin corresponding to dynamin1 spots is significantly higher than dynamin1 outside clathrin spots.