Ldb19 co-localizes with TGN clathrin adaptors.

(A-D) Cells expressing indicated fusion proteins from their endogenous loci were imaged and analyzed for co-localizationArrows indicate co-localization, filled and outlined arrowheads indicate structures with only mCh or GFP, respectively, and double arrows indicate cell surface-localized Ldb19-GFP structures. Right, quantification of the percentage of Ldb19-GFP fluorescent structures that contained mCh fluorescence (black bar) and the percentage of mChfluorescent structures that contained GFP fluorescence (white bar). Error bars indicate standard deviation from the mean of three separate experiments (n = 3), each with at least 200 puncta and 70 cells. (E) Kymograph showing dynamic recruitment of Ldb19-GFP and Apl4-mCh. Top: Images of structures analyzed by live-cell microscopy. Boxed region indicate structures selected for Kymograph. Bottom: kymographs of the boxed area in the top images is shown; the boxed area indicates the time point shown above.