Increase in dynamin1 intensity at sites of clathrin-mediated endocytosis in PC12 cells.

<p>PC12 cells co-transfected with clathrin-dsRed (A) and dynamin1-EGFP (B) were imaged by live-cell TIR-FM. Frames with time-stamps represent magnifications of regions identified directly to the left. Quantification of the spot marker by the arrow (C) shows burst of dynamin1 fluorescence just prior to endocytosis. Additionally, clathrin fluorescence remains roughly constant prior to internalization. Quantification of 12 such events is shown is (D).