Immunohistochemistry in the dentate gyrus (DG) of wild-type and <i>Rev-erbα^−/−</i> mice at ZT6.

<p>(<b>A</b>) Cell nuclei stained with DAPI are in blue and antibodies recognizing doublecortin (Dcx) are in red. Dcx expression is increased in the subgranular zone of <i>Rev-erbα^−/−</i> mice indicating the presence of more neuroblasts in these animals. Scale bar: 100 µm. (<b>B</b>) Left panel: Visualization of cell division using bromodeoxyuridine (BrdU). Antibodies recognizing NeuN in blue mark nuclei of mature neurons, antibodies recognizing Dcx are in red and antibodies against BrdU are in green. Scale bar: 50 µm. Right panel: Quantification of the BrdU⁺ cells after 4 days. <i>Rev-erbα^−/−</i> mice display more BrdU positive cells (mean ± SEM, n = 3, **p<0.005, t-test). (<b>C</b>) FABP7 protein expression (green) does not overlap with Dcx protein expression and both expression levels are higher in <i>Rev-erbα^−/−</i> mice. The orthogonal sectioning to the right and at the bottom show reconstructions from a confocal z-stack in xz and yz direction, respectively. The dotted white lines mark the granular layer of the DG. Scale bar: 50 µm.