Analysis of cell proliferation and neurogenesis in the dentate gyrus of experimental groups.

<p>(A-L) Low-power photomicrographs illustrating BrdU-positive (A-D) and double-labeled BrdU/NeuN-positive cells (E-L) 20 days after the accumulative BrdU labeling in AL-n (A,E,I), PHY-n (B,F,J), SO-n (C,G,K), and SOPHY-n (D,H,L) groups. Notice the increased number of BrdU- and double-labeled cells in the SOPHY-n and PHY-n groups. A higher magnification of BrdU/NeuN-labeled cells in the suprapyramidal blade of the dentate gyrus of each experimental group is shown in I-L. (N) Example of a double-labeled cell in the subgranular zone (SGZ) of a mouse from the SOPHY-n group. The X and Y orthogonal projections of the photomicrograph are displayed (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011130#s4" target="_blank">Material and Methods</a> for details). (O-P) Histograms showing the number (mean ± s.e.m.) of BrdU-positive cells (O) and double-labeled cells (BrdU-NeuN) in the SGZ + GL of the dentate gyrus in experimental groups. Asterisks indicate statistical differences between groups. (<i>P</i>≤0.001; ANOVA). Abbreviations: GL, granule cell layer; H, Hilus; ML, molecular layer. Scale bars: A = 200 µm pertains to B-H; I = 100 µm pertains to J-L, N = 10 µm.