Bromodeoxyuridine (BrdU) cell proliferation tracking in piglet OB SVZ.

<p><b>A</b> and <b>B</b>. Confocal microscope images showing that the OB contains numerous BrdU⁺ cells (A, green) and subsets of these cells are newly born neurons as shown by the colocalization (B, seen as yellow, arrows) with the neuron marker NeuN. <b>C</b>. Confocal microscope images showing the co-labeling (arrows) of the DNA synthesis marker BrdU (red) and glial fibrillary acidic protein (GFAP, green) in the OB SVZ. Some BrdU⁺ nuclei are associated with GFAP⁺ cytoplasm. Scale bars = 33 µm (A), 17 µm (B), 20 µm (C). <b>D</b>. Graph showing the proportions of the total BrdU⁺ cells that are either GFAP⁺ or NeuN⁺. Values are mean ± SD (n=4). Some BrdU⁺ cells were not positive for either marker. Asterisk denotes NeuN significant difference (p < 0.001) compared to GFAP.