Purification of rPbPga1 expressed in <i>P. pastoris</i>.

<p>Supernatant samples from induced <i>Pp</i>CV1 cells were analyzed by (A) silver staining of SDS-PAGE gels and by (C) Western blot using an anti-His antibody (1∶1000). The supernatant (S) from induced <i>Pp</i>CV1 cells was subjected to a nickel column and eluted samples were analyzed (B) by silver stained SDS PAGE gel and (D) Western blot using anti-His antibody (1∶1000). A polypeptide of 74 kDa corresponding to rPbPga1 was detected in all samples (arrows). Flow through sample (FT). Unbound washed sample (W1 and W4). Resin bound rPbPga1 (R).