Western blot using anti-His<sub>6</sub> primary antibody showing expression and purification of recombinant (A) AgTx2 and (B) MgTx produced in <i>P. pastoris</i>.
- Raveendra Anangi (292358)
- Shyny Koshy (53125)
- Redwan Huq (292360)
- Christine Beeton (292361)
- Woei-Jer Chuang (189690)
- Glenn F. King (233183)
Publication date
February 2013
DOIAbstract
<p>The mass (in kDa) of the prestained molecular weight markers (“Stds”) is indicated. Lane 1, culture supernatant after methanol induction of toxin expression; Lane 2, recombinant peptide after purification using nickel affinity chromatography; Lane 3 recombinant peptide after RP-HPLC purification.</p
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