The expression and purification of rhSCF^1–141 and rhSCF^1–165.

<p>(A) PCR analysis of pPICZαC-rhSCF^1–141 (1011 bp) and pPICZαC/SCF^1–165 (1083 bp) using <i>5′AOX I</i> and <i>3′AOX I</i> primers (arrow). (B) Coomassie blue-stained SDS-PAGE of rhSCF^1–141 and rhSCF^1–165overexpressed from <i>Pichia</i> culture supernatants. Lane M: molecular weight markers. Numbers above the lanes represent hours after methanol induction. Arrows indicate the overexpressed rhSCF^1–141 or rhSCF^1–165 (C) Silver-stained 15% SDS–PAGE of purified rhSCF^1–141 and rhSCF^1–165 produced in <i>P. pastoris</i>. Lane M, molecular weight markers. Lanes 1 and 2, purified rhSCF^1–141 (200 and 100 ng, respectively); Lanes 3 and 4, purified rhSCF^1–165 (20 and 50 ng, respectively). (D) Western blot analysis of rhSCF^1–141-6His and rhSCF^1–165-6His from culture supernatants. The recombinant proteins were 6xHis tagged and detected with anti-His antibodies.