Co-sedimentation among paralogs.
- María José Castelló (530455)
- Laura Medina-Puche (4284865)
- Julián Lamilla (4284862)
- Pablo Tornero (125801)
Publication date
December 2018
DOI Publisher
Public Library of Science (PLoS)Abstract
(a) GFP-NPR2 and the MBP fused to some NPR1 paralogs were transiently expressed in N. benthamiana by agroinfiltration and pulled-down with amylose resin. MBP-AKIN10 [37] is an unrelated protein, used as negative control with a similar size to the MBP-NPRs. The panel shows GFP-NPR2 detected by immunoblot before treatment with resin. Ponceau-S staining of the nitrocellulose membrane is shown as a loading control. (b) Expression of MBP-NPRs detected by immunoblot analysis before treatment with resin. (c) The panel shows the eluted fraction from the resin detecting GFP-NPR2 by immunoblot analysis. (d) The same eluted fraction detecting MBP-NPRs.</p
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