PAGE and immunoblot analysis of the purification process of recombinant HP-NAP expressed in <i>B. subtilis</i>.

<p>The whole cell lysate (W) and soluble fraction (S) of <i>B. subtilis</i> DB104-pRPA-NAP, HP-NAP-containing flow-through fractions (F) obtained by DEAE Sephadex chromatography, and the concentrated HP-NAP (C) from flow-through fractions were analyzed by SDS-PAGE (<b>A</b>), native-PAGE (<b>B</b>), and immunoblotting (<b>C</b>) with an anti-HP-NAP antibody (MAb 16F4). Molecular weights (M) in kDa are indicated on the left of stained gels and the blot.