Polymerase sub-complexes lacking Rpb1 accumulate in <i>not5Δ</i>.

<p><b>A</b> and <b>B</b>. Total extracts from cells expressing the indicated Tap-tagged (TT) polymerase subunits were separated on native gels (upper panels) or SDS-PAGE (lower panels) and analyzed by western blotting with anti- CBP antibodies. <b>C</b>. Rpb9-TT was purified by single step affinity and the purified proteins were analyzed on native gels (upper panels) or SDS-PAGE (lower panels) and western blotting with anti-CBP antibodies (left panel) or anti-Rpb1 antibodies (right panel). <b>D</b>. Total extracts from cells expressing Rpb11-TT were either untreated (-) or treated with DNase or RNase as indicated and separated by Native-PAGE, and analyzed by western blotting with PAP antibodies.