Lack of Bud27 affects assembly of RNA pol I, II, and III.

<p>A) RNA pol II was immunoprecipitated with anti-Rpb1 antibodies (8WG16) from a strain containing Rpb2-TAP, Rpb3-HA and Rpb4-Myc. RNA pol II subunits were analysed with Anti-HA, anti-Rpb1 (8WG16), anti-Myc, anti-PAP, anti-Rpb5 and anti-Rpb6 antibodies. B) Western blot of Rpb1 co-purified with RPB2-TAP. Anti-Rpb1 (8WG16) and anti-PAP antibodies were used. C) RNA pol I, II and III were immunoprecipitated with anti-HA, anti-Rpb1 (8WG16) and anti-Myc antibodies from a wild-type and a Δ<i>bud27</i> mutant strains containing Rpa190-HA (RNA pol I) and Rpc160-Myc (RNA pol III), transformed with a plasmid overexpressing RPB5 (<i>pFL-RPB5</i>), or with an empty vector (<i>pFL</i>). Rpa190-HA, Rpb1, Rpc160-Myc, Rpb5, and Rpb6 were analysed by Western blot with the antibodies indicated above.