SDS-PAGE (A) and Western blotting (B) analysis of rMp1p expressed in <i>Pichia pastoris</i>.
- Yan-Fang Wang (80112)
- Jian-Piao Cai (336878)
- Ya-Di Wang (336879)
- Hui Dong (153701)
- Wei Hao (134390)
- Ling-Xiao Jiang (336880)
- Jun Long (336881)
- Cheman Chan (336882)
- Patrick C. Y. Woo (141255)
- Susanna K. P. Lau (141251)
- Kwok-Yung Yuen (2377228)
- Xiao-Yan Che (336883)
Publication date
February 2013
DOIAbstract
<p>(A) M: protein molecular-mass marker; Lane 1: purified rMp1p; Lane 2 to 4: induced supernatant of positive transformant (pPIC9K-Mp1p/GS115) at 3, 4 and 5 days, respectively; Lane 5: induced supernatant of control transformant (pPIC9K/GS115); (B) Lane 1: the serum from a guinea pig immunized with rMp1p from <i>E.coli</i>; Lane 2: the serum from guinea pigs pre-immunization; Lane 3: irrelevant MAb control; Lane 4: anti-His MAb.</p
Add to ORKG