Effect of TRPM7 inhibition on HUVEC migration and NO production.

<p>HUVEC were treated with 2-APB and Co(III)hexaammine or transfected with a shRNA against <i>TRPM7</i> for 24 h. (A) The cells were wounded and migration was allowed for 10 h. Microphotographs show the result of a representative assay (20×magnification). (B) The wound area was calculated by ImageJ software and expressed using an arbitrary value scale to quantify the results. The values are expressed as the mean of 3 different experiments ± standard deviation. *p<0.05, **p<0.01. (C) NOS activity was measured by using the Griess method.