The effects of shRNA-VCAM-1 alone or combined with artemether on the invasiveness of U87 cells.

<p>(A) U87 cells were treated with artemether of 300 µmol/L, shRNA transfected or treated with both. Serum free L-DMEM served as a negative control. Cells migrating to the underside surface of Transwell filter were stained with Giemsa staining and photomicrographed, representing the invasiveness ability of U87 cells. (×400). (B) Statistical analysis of invasive cell numbers. The invasiveness of shRNA-VCAM-1 U87 cells was inhibited significantly compared with control group and the artemether+shRNA-VCAM-1 group displayed the maximum inhibitory effects. <i>n</i> = 4. *<i>P</i><0.01 (compared with the control group); ^#<i>P</i><0.01, (compared with artemether group); ^×<i>P</i><0.01 (compared with shRNA-VCAM-1 group).