TRPM8 inhibition impairs migration of prostate tumor cells.

<p>Wound healing assays were performed on PNT1A, LNCaP, PC3 and DU145 cells in the absence or the presence of the TRPM8 inhibitors AMTB and JNJ41876666 (10 µM). A. Representative images of experiments performed in the presence of normal serum concentrations. In the absence of blocker, all cell lines efficiently restore the monolayer after 24 (LNCaP and DU145) or 48 h (PNT1A; at 24 h, no recovery was observed). The presence of blockers impairs healing in LNCaP, PC3 and DU145, but not in PNT1A cells. B. Similar effects were observed on all cell lines already 12 h after the scratch. C. Quantification of restored surface from three images like those in A, for 24 h in all cell lines, except PNT1A (48 h) in the presence of JNJ41876666 (black) and AMTB (red). D. Similar experiments in low serum concentrations revealed that PNT1A did not reduce the scratch surface, while cancer cells LNCaP and PC3 (in 3% FCS) and DU145 (in 1% FCS) did. Healing was again inhibited by JNJ41876666 (10 µM). E. Menthol induced a slight acceleration of healing only in DU145 cells, while icilin showed no effect on any of the lines tested.