Selective inhibitory effect of TRPM7 blockade on activated T cell migration.

<p>A. Lack of effect of SKF-96365 on TRPM7 currents. Representative TRPM7 currents in activated T cells were recorded before (control) and after extracellular application of 20 µM SKF-96365. Currents were induced from a HP of 0 mV by ramp depolarization from −100 mV to +100 mV every 10 s. The average current amplitude (lower panel) for TRPM7 channels was calculated at +100 mV (n = 8). B. Effect of SKF-96365 (20 µM) on T cell migration. T cell migration was measured in the same cell before and after treatment with the blocker. The data are the average of 18 cells from 2 individuals. C. Inhibition of native TRPM7 currents by 2-APB. Representative TRPM7 currents in activated T cells were recorded before (control) and after increasing concentrations of 2-APB. Currents were elicited like in A. The corresponding average current amplitudes are shown in the bottom panel (n = 7). D. Effect of 2-APB on T cell migration. T cell migration was measured in the same cell before and after treatment with progressive concentrations of 2-APB. The data are the mean of 15 cells from 2 individuals. Statistical significance in panels C and D was determined by One Way Repeated Measures ANOVA and post-hoc testing for significance was determined by Holm-Sidak method. * represents statistical significance in the treatment groups as compared to the control.