Effect of injured RPE cells on HUVEC migration.

<p>HUVEC migration was examined with a phase-contrast microscopy (×40) at the indicated time points (0h, 6h, 10h)(A). The wounding width was quantified, and bars represent the migration index of each treatment. The assay showed a significant increase of the distance moved by HUVECs treated with human serum that was cultured with H₂O₂-damaged ARPE-19 cells, when compared to HUVECs treated with the serum that was cultured with control ARPE-19 cells (P < 0.05) (B). The data were expressed as mean ± SD from results done with three independent biological samples, each analyzed with triplicates.