Gene expression of macrophage type distribution in mouse retroperirenal adipose depot and monocyte infiltration response to adipose tissue conditionated-media adipokine.

<p>Retroperirenal adipose tissue mRNA expressions of F4/80 (total macrophage, A), CD11c (type M1, B) and CD163 (type M2, C) in Ctl (circle and dotted line) and C5L2^−/− (square and solid line) on chow diet (white circle or square) or DIO (black circle of square) over 6, 12 and 24 weeks. Data are expressed as relative expression (re) mean±SEM (n = 4–5 mice per group) compared to 6 weeks, chow diet Ctl set as 1. All data were analysed with two-way (2-way) ANOVA for genotype × time between Ctl and C5L2^−/− for each diet or adipose tissue with a Bonferroni post-test where * <i>p</i><0.05 and ** <i>p</i><0.001 for Ctl vs. C5L2^−/− for the same diet or tissue. Monocyte migration was assessed in bone marrow monocytes isolated from Ctl and C5L2^−/− mice. Cells were incubated without treatment (Basal) or with conditioned media (CM) derived from gonadal adipose tissue from Ctl (Ctl CM) or C5L2^−/− (C5L2^−/− CM) mice (D). Data are expressed as relative expression (re) of monocyte migration mean±SEM (n = 3–5 migration experiments per condition). The data were analysed with regular one-way ANOVA with Bonferroni multiple comparisons test, where “&” indicates significant difference from basal condition, “#” indicates significant difference from Ctl CM and “*” indicate significant difference between the genotype of monocyte for the same condition, all <i>p</i><0.05. Additional 2-way ANOVA for diet × time for F4/80 expression indicates a diet effect in retroperirenal (A) for Ctl (<i>p</i> = 0.0001) and C5L2^−/− (<i>p</i><0.0001).