Recruitment of macrophages into adipose tissue and immunogenic phenotype of macrophages isolated from adipose tissue.

<p>Adipose tissue was harvested from control and <i>Aah</i>-injected mice after 24 hours and stromal vascular cells (SVCs) were separated from tissue by collagenase digestion. SVCs were stained for F4/80, CD11b, and CD11c cell surface proteins and analyzed by flow cytometry. (A) Representative flow-cytometry profiles for the expression of M1 Macrophages (F4/80 high CD11b high CD11b+) from control and <i>Aah</i>-injected mice. Numbers indicate the percentages of M1 cells found in the indicated gates. Histograms show the quantifications of the percentages of M1 macrophages. (B) Representative flow-cytometry profiles for M2 macrophages (F4/80 high CD11c high CD11c negatif) from control and <i>Aah</i>-injected mice. Numbers indicate the percentage of cells found in the indicated gates. (C) Histograms show the quantifications of the percentage of F4/80 high alone, CD11b high alone, and CD11c positive cells. The means, standard deviations, and numbers of mice are indicated. Analyses were by two-way ANOVA,*, p<0.05.