Flow cytometric analyses of infiltrated Mφ in perigonadal adipose tissue.

<p>(A) A representative flow cytometric dot-plot defining the F4/80⁺CD11b⁺ population in perigonadal adipose tissue in HFD-fed mice. F4/80^hi (Population 1; red gate) and F4/80^low∼− (Population 2; blue gate) are set to a staining with isotype control mAb. (B) The percentage of cells of CD11b⁺ (total) (a), Population 1 (b) and Population 2 (c). (C) CD1d expression by the Population 1 and Population 2 (a), MFI in WT (b) and Jα18^−/− (c) mice fed an HFD (n = 3–4 male mice in each group). The results are expressed as mean ± s.d. Statistical analysis was performed according to the Tukey-Kramer test. *<i>p</i><0.05, **<i>p</i><0.01.