Flow cytometry analysis of F4/80 and CD11c (M1 pro-inflammatory) macrophage markers in mouse inguinal and perigonadal adipose depots.

<p>Percentage of macrophages based on F4/80 (A and B) and CD11c (C and D) markers in inguinal (A–C) and perigonadal (B–D) stromal vascular fraction (SVF) of adipose tissue after 6, 12 and 24 weeks of chow diet (white circle or square) and DIO (black circle or square) for Ctl (circle and dotted line) and C5L2^−/− (square and solid line) mice. Data are presented as percentage of F4/80 positive cells (total macrophage) over total cells of the SVF (A–B) or as CD11c percent of positive cells relative to total macrophages (C–D) as mean±SEM (n = 4–8 adipose tissues per group). All data were analysed with two-way (2-way) ANOVA with genotype × time between Ctl and C5L2^−/− for each adipose tissue with a Bonferroni post-test where * <i>p</i><0.05 and ** <i>p</i><0.01 for Ctl vs. C5L2^−/− for the same diet in the same tissue. Additional 2-way ANOVA for diet × time for % macrophages indicates a diet effect in inguinal (A) for Ctl (<i>p</i> = 0.028) and C5L2^−/− (<i>p</i> = NS) and in perigonadal (B) for Ctl (<i>p</i> = 0.05) and C5L2^−/− (<i>p</i> = 0.01).